PPP2R5D antibody (C-Term)

Catalog No. ABIN374132

The Goat Polyclonal anti-PPP2R5D antibody is suitable to detect PPP2R5D in samples from Human, Mouse, Rat, Cow, Dog and Pig. It has been validated for WB.

Quick Overview for PPP2R5D antibody (C-Term) (ABIN374132)

Target: PPP2R5D (Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R5D antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPP2R5D Antibody

Binding Specificity: C-Term

Sequence: C-KRAEEFLTAS QEAL

Specificity: This antibody detects PPP2R5D at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_006236.1, NP_851307.1, NP_851308.1 Genename: PPP2R5D

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 20000. Western blot: 1-3 μg/mL. Approx 70 kDa and 60 kDa bands seen in 293 lysate (Predicted MWof 60 kDa and 60 kDa according to NP_006236 and AAH01175, respectively).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PPP2R5D

Target: PPP2R5D (Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D))

Alternative Name: PPP2R5D

Background: Synonyms: PP2A B subunit B'delta isoform, PP2A B subunit B56 delta isoform, PP2A B subunit PR61 delta isoform, PP2A B subunit R5 delta isoform, Protein phosphatase 2A, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform

Gene ID: 5528

NCBI Accession: NP_006236

UniProt: Q14738

Pathways: PI3K-Akt Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades