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PYD and CARD Domain Containing (PYCARD) (C-Term) antibody

Details for Product No. ABIN374154
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Antigen
Synonyms ASC, CARD5, TMS, TMS-1, TMS1, Asc
Epitope
C-Term
(15), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(63), (21), (18), (12), (12), (12), (8), (1)
Host
Goat
(50), (12), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(57), (24), (17), (11), (10), (9), (6), (5), (3), (3), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN374154
357.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide from C Terminus of the protein sequence according to NP_037390.2, NP_660183.1 Genename: PYCARD
Sequence C-RESQSYLVEDLERS
Specificity This antibody is expected to recognize all reported Human isoforms of PYCARD according to NP_037390.2 and NP_660183.1. Species Reactivity: Tested: Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name CARD5 / PYCARD / ASC
Background TMS1 (target of methylation-induced silencing) or ASC (apoptosis-associated speck-like protein containing a CARD) promotes caspase-mediated apoptosis. TMS1 is aberrantly methylated and silenced in human breast cancer cells, the methylation-mediated silencing of TMS1 may confer a survival advantage by allowing cells to escape from apoptosis, supporting a new role for aberrant methylation in breast tumorigenesis. TMS1 encodes a 22-kDa predicted protein containing a COOH-terminal caspase recruitment domain (CARD). However, it is structurally unrelated to other known CARD adaptor and regulatory proteins. Ectopic expression of TMS1 alone can trigger apoptosis in 293 cells, and cell death correlated with relocalization of ASC from the cytoplasm to perinuclear, ball- like structures.
Alternate names: Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, TMS1, Target of methylation-induced silencing 1, poptosis-associated speck-like protein containing a CARD
Gene ID 29108
NCBI Accession NP_037390.2
UniProt Q9ULZ3
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 µg/ml. Approx.22kDa band observed in Hela and Human Kidney lysates(predicted MW of 22kDa according to NP_037390.2). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-PYD and CARD Domain Containing (PYCARD) (C-Term) antibody anti-PYD and CARD Domain Containing (PYCARD) (C-Term) antibody
anti-PYD and CARD Domain Containing (PYCARD) (C-Term) antibody (2) anti-PYD and CARD Domain Containing (PYCARD) (C-Term) antibody (Image 2)
anti-PYD and CARD Domain Containing (PYCARD) (C-Term) antibody (3) anti-PYD and CARD Domain Containing (PYCARD) (C-Term) antibody (Image 3)
Background publications Ohtsuka, Ryu, Minamishima et al.: "ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway." in: Nature cell biology, Vol. 6, Issue 2, pp. 121-8, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN374154
357.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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