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PYD and CARD Domain Containing (PYCARD) (C-Term) antibody

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Synonyms ASC, CARD5, TMS, TMS-1, TMS1, 9130417A21Rik, Asc, TNS1, masc, asc1, zAsc, zgc:109874
(28), (13), (8), (7), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
(99), (13), (10), (1), (1), (1), (1)
(62), (31), (14)
(4), (4), (4), (4), (3), (3)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(97), (49), (23), (17), (10), (8), (7), (3), (3), (2)
Pubmed 1 reference available
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Quantity 0.1 mg
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Immunogen Peptide from C Terminus of the protein sequence according to NP_037390.2, NP_660183.1 Genename: PYCARD
Specificity This antibody is expected to recognize all reported Human isoforms of PYCARD according to NP_037390.2 and NP_660183.1.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name CARD5 / PYCARD / ASC (PYCARD Antibody Abstract)
Background TMS1 (target of methylation-induced silencing) or ASC (apoptosis-associated speck-like protein containing a CARD) promotes caspase-mediated apoptosis. TMS1 is aberrantly methylated and silenced in human breast cancer cells, the methylation-mediated silencing of TMS1 may confer a survival advantage by allowing cells to escape from apoptosis, supporting a new role for aberrant methylation in breast tumorigenesis. TMS1 encodes a 22- kDa predicted protein containing a COOH-terminal caspase recruitment domain (CARD). However, it is structurally unrelated to other known CARD adaptor and regulatory proteins. Ectopic expression of TMS1 alone can trigger apoptosis in 293 cells, and cell death correlated with relocalization of ASC from the cytoplasm to perinuclear, ball-like structures.Synonyms: Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, TMS1, Target of methylation-induced silencing 1, poptosis-associated speck-like protein containing a CARD
Gene ID 29108
NCBI Accession NP_037390
UniProt Q9ULZ3
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx.22 kDa band observed in Hela and Human Kidney lysates(predicted MW of 22 kDa according to NP_037390.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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Background publications Ohtsuka, Ryu, Minamishima et al.: "ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway." in: Nature cell biology, Vol. 6, Issue 2, pp. 121-8, 2004 (PubMed).

Catalog No. ABIN374154
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