PYCARD antibody (C-Term)

Catalog No. ABIN374154

Product Details anti-PYCARD Antibody

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-RESQSYLVED LERS

Specificity: This antibody is expected to recognize all reported Human isoforms of PYCARD according to NP_037390.2 and NP_660183.1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_037390.2, NP_660183.1 Genename: PYCARD

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx.22 kDa band observed in Hela and Human Kidney lysates(predicted MW of 22 kDa according to NP_037390.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: CARD5 / PYCARD / ASC (PYCARD Products)

Synonyms: ASC antibody, CARD5 antibody, TMS antibody, TMS-1 antibody, TMS1 antibody, 9130417A21Rik antibody, Asc antibody, TNS1 antibody, masc antibody, asc1 antibody, zAsc antibody, zgc:109874 antibody, PYD and CARD domain containing antibody, PYCARD antibody, Pycard antibody, pycard antibody

Background: TMS1 (target of methylation-induced silencing) or ASC (apoptosis-associated speck-like protein containing a CARD) promotes caspase-mediated apoptosis. TMS1 is aberrantly methylated and silenced in human breast cancer cells, the methylation-mediated silencing of TMS1 may confer a survival advantage by allowing cells to escape from apoptosis, supporting a new role for aberrant methylation in breast tumorigenesis. TMS1 encodes a 22- kDa predicted protein containing a COOH-terminal caspase recruitment domain (CARD). However, it is structurally unrelated to other known CARD adaptor and regulatory proteins. Ectopic expression of TMS1 alone can trigger apoptosis in 293 cells, and cell death correlated with relocalization of ASC from the cytoplasm to perinuclear, ball-like structures.Synonyms: Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, TMS1, Target of methylation-induced silencing 1, poptosis-associated speck-like protein containing a CARD

Gene ID: 29108

NCBI Accession: NP_037390

UniProt: Q9ULZ3

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome