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MAP/microtubule Affinity-Regulating Kinase 4 (MARK4) (C-Term) antibody

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Synonyms MARK4, MARK4L, MARK4S, MARKL1, MARKL1L, PAR-1D, 2410090P21Rik, C79806, Markl1
(47), (21), (15), (13), (7), (2), (2), (1), (1), (1), (1), (1)
(98), (57), (30)
(99), (15), (1)
(9), (9), (9), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Western Blotting (WB)
(70), (58), (22), (21), (14), (10), (4), (2), (1)
Pubmed 1 reference available
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Quantity 0.1 mg
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Immunogen Peptide from the C Terminus of the protein sequence according to NP_113605.2. Genename: MARK4
Specificity This antibody detects MARK4 at C-term.
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name MARK4 (MARK4 Antibody Abstract)
Background MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells.Synonyms: MAP microtubule affinity-regulating kinase 4, MARKL1
Gene ID 57787
NCBI Accession NP_113605
UniProt Q96L34
Research Area Signaling, Cytoskeleton
Application Notes Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.3-1 μg/mL. Approx 70 kDa band observed in human prostate lysates(calculated MW of 75.0 kDa according to NP_113605.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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 image for anti-MAP/microtubule Affinity-Regulating Kinase 4 (MARK4) (C-Term) antibody (ABIN374191) anti-MAP/microtubule Affinity-Regulating Kinase 4 (MARK4) (C-Term) antibody
Background publications Kato, Satoh, Okabe et al.: "Isolation of a novel human gene, MARKL1, homologous to MARK3 and its involvement in hepatocellular carcinogenesis." in: Neoplasia (New York, N.Y.), Vol. 3, Issue 1, pp. 4-9, 2001 (PubMed).

Catalog No. ABIN374191
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