MARK4 antibody (C-Term)

Catalog No. ABIN374191

This Goat Polyclonal antibody specifically detects MARK4 in WB. It exhibits reactivity toward Human.

Quick Overview for MARK4 antibody (C-Term) (ABIN374191)

Target: MARK4 (MAP/microtubule Affinity-Regulating Kinase 4 (MARK4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MARK4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARK4 Antibody

Binding Specificity: C-Term

Sequence: IKRKPPPGCS DSPGV

Specificity: This antibody detects MARK4 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_113605.2. Genename: MARK4

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.3-1 μg/mL. Approx 70 kDa band observed in human prostate lysates(calculated MW of 75.0 kDa according to NP_113605.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for MARK4

Target: MARK4 (MAP/microtubule Affinity-Regulating Kinase 4 (MARK4))

Alternative Name: MARK4

Background: MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells.Synonyms: MAP microtubule affinity-regulating kinase 4, MARKL1

Gene ID: 57787

NCBI Accession: NP_113605

UniProt: Q96L34