JUP antibody (C-Term)

Catalog No. ABIN374233

This Goat Polyclonal antibody specifically detects JUP in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow and Pig.

Quick Overview for JUP antibody (C-Term) (ABIN374233)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-JUP Antibody

Binding Specificity: C-Term

Sequence: C-GLRPPYPTAD HMLA

Specificity: This antibody detects Junction plakoglobin at C-term. This antibody is expected to recognise both reported isoforms (NP_002221.1 and NP_068831.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine (Dog), Pig.
Species reactivity (tested):Human, Rat.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002221.1, NP_068831.1 Genename: JUP

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 85 kDa band observed in A431 lysate (predicted MW of85 kDa according to NP_002221, NP_068831).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Junction Plakoglobin

Background: Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.Synonyms: CTNNG, Catenin gamma, DP3, Desmoplakin III, Desmoplakin-3, JUP

Gene ID: 3728

NCBI Accession: NP_002221

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location