CDT1 antibody (C-Term)

Catalog No. ABIN374262

The Goat Polyclonal anti-CDT1 antibody (ABIN374262) specifically detects CDT1 in WB. The antibody is reactive with Human samples.

Quick Overview for CDT1 antibody (C-Term) (ABIN374262)

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CDT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDT1 Antibody

Binding Specificity: C-Term

Sequence: C-ARLAHQTRAE EGL

Specificity: This antibody detects CDT1 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_112190.2. Genename: CDT1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western Blot: 1-3 μg/mL. Approx 65 kDa band observed in Human Brain lysates (predictedMW of 65 kDa according to NP_112190.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for CDT1

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Alternative Name: CDT1

Background: Cdt1 and its inhibitor Geminin are important regulators of replication licensing. A critical balance between both proteins functions to ensure that each origin only fires once during the cell cycle. Cdt1 cooperates with Cdc6 to promote the loading of the MCM complex onto chromatin to form the pre-replication complex. Overexpression of Cdt1 leads to aberrant replication and activation of DNA damage checkpoints. It is also associated with a predisposition for malignant transformation.Synonyms: DNA replication factor Cdt1, DUB, Double parked homolog, RIS2

Gene ID: 81620

NCBI Accession: NP_112190

UniProt: Q9H211

Pathways: MAPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA