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POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibody

Details for Product No. ABIN374274, Supplier: Log in to see
Antigen
  • BOB1
  • OBF-1
  • OBF1
  • OCAB
  • BOB-1
  • BOB.1
  • Bob-1
  • Bob1
  • OBF.1
  • OCA-B
Epitope
C-Term
43
19
9
9
2
2
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
125
43
37
6
5
1
Host
Goat
81
38
15
Clonality
Polyclonal
Conjugate
Un-conjugated
9
9
9
5
5
5
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
93
54
32
27
22
17
3
2
1
1
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Immunogen Peptide from C Terminus of the protein sequence according to NP_006226.2 Genename: POU2AF1
Sequence C-DAYALNHTLS VEGF
Specificity This antibody detects POU2AF1 / BOB1 at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Dog, CowSpecies reactivity (tested):Human, Mouse
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name POU2AF1 / BOB1 (POU2AF1 Antibody Abstract)
Background Synonyms: B-cell-specific coactivator OBF-1, OBF-1, OBF1, OCA-B, OCAB, OCT-binding factor 1, POU domain class 2-associating factor 1
Gene ID 5450
NCBI Accession NP_006226
UniProt Q16633
Pathways
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30 kDa band seen in mouse spleen lysate (Predicted MW of20 kDa according to NP_006226).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibody (ABIN374274) anti-POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibody
Background publications Strubin, Newell, Matthias: "OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins." in: Cell, Vol. 80, Issue 3, pp. 497-506, 1995 (PubMed).