NDRG1 antibody (C-Term)

Catalog No. ABIN374281

This Goat Polyclonal antibody specifically detects NDRG1 in WB. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for NDRG1 antibody (C-Term) (ABIN374281)

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This NDRG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NDRG1 Antibody

Binding Specificity: C-Term

Sequence: GNSAGPKSME VSC

Specificity: This antibody detects NDRG1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006087.2, NP_001128714.1 Genename: NDRG1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 48 kDa band observed in lysates of Human Brain,Kidney and Testes (calculated MW of 42.8 kDa according to NP_006087.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NDRG1

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Alternative Name: NDRG1

Background: This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom. May have a growth inhibitory role. Ubiquitous, expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.Synonyms: CAP43, DRG-1, DRG1, Differentiation-related gene 1 protein, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, RTP, Reducing agents and tunicamycin-responsive protein, Rit42

Gene ID: 10397

NCBI Accession: NP_006087

UniProt: Q92597