SETDB2 antibody (N-Term)

Catalog No. ABIN374283

The Goat Polyclonal anti-SETDB2 antibody (ABIN374283) specifically detects SETDB2 in WB and EIA. The antibody is reactive with Human, Rat, Mouse, Cow, Dog and Pig samples.

Quick Overview for SETDB2 antibody (N-Term) (ABIN374283)

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Reactivity: Human, Rat, Mouse, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This SETDB2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SETDB2 Antibody

Binding Specificity: N-Term

Sequence: GEKNGDAKTF WME-C

Specificity: This antibody is expected to recognize both reported isoforms (NP_114121.2, NP_001153780.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Porcine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide (GEKNGDAKTFWME-C) from the N-Terminus of the protein sequence according to NP_114121.2, NP_001153780.1. Genename: SETDB2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 1000. Western blot: 0.3-1 μg/mL. Approx 80 kDa band observed in human heart lysates(calculated MW of 81.0 kDa according to NP_114121.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SETDB2

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Alternative Name: SETDB2 / CLLD8

Background: Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity.Synonyms: C13orf4, Chronic lymphocytic leukemia deletion region gene 8 protein, Histone-lysine N-methyltransferase SETDB2, KMT1F, Lysine N-methyltransferase 1F, SET domain bifurcated 2

Molecular Weight: 81.9 kDa (NP_114121.1)

Gene ID: 83852

NCBI Accession: NP_114121

UniProt: Q96T68