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Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibody

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Synonyms e2-25k, hip2, hypg, lig, HIP2, E2-25K, HYPG, LIG, UBC1, AW492011, D5Ertd601e, E2-25k, Hip2, Hypg, Lig, ube2k, zgc:103472
(24), (18), (15), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine)
(110), (31), (22), (11), (10), (9), (6), (6), (6), (4)
(81), (19), (9), (2)
(8), (8), (8), (3), (3), (3), (1)
Western Blotting (WB)
(93), (54), (29), (9), (4), (3), (3), (3), (2), (1), (1)
Pubmed 1 reference available
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Quantity 0.1 mg
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Immunogen Peptide from C Terminus of the protein sequence according to NP_005330.1, NP_001104582.1, NP_001104583.1. Genename: UBE2K
Specificity This antibody recognizes UBE2K/HIP2 (C-term). It is expected to recognise all reported isoforms.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Canine (Dog), Pig and Cow.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name HIP2 / UBE2K (UBE2K Antibody Abstract)
Background The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. HIP2 belongs to the ubiquitin conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short lived and abnormal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid bearing macrophages through ubiquitination and subsequent degradation of p53.Synonyms: E2(25K), E2-25K, HIP-2, Huntingtin-interacting protein 2, LIG, Ubiquitin carrier protein, Ubiquitin-conjugating enzyme E2 K, Ubiquitin-protein ligase
Gene ID 3093
NCBI Accession NP_005330
UniProt P61086
Application Notes Peptide ELISA: Limit Dilution: 1/2000. Western blot: 1-3 μg/mL. Approx 24 kDa band observed in Jurkat cell lysates (CalculatedMW of 22.4 kDa according to NP_005330).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted at (in aliquots) at -20 °C.
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 image for anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibody (ABIN374290) anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibody
Background publications Kalchman, Graham, Xia et al.: "Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme." in: The Journal of biological chemistry, Vol. 271, Issue 32, pp. 19385-94, 1996 (PubMed).

Catalog No. ABIN374290
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