FOXA2 antibody (C-Term)

Catalog No. ABIN374335

Product Details anti-FOXA2 Antibody

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-GVYSRPIMNS S

Specificity: This antibody detects FOXA2 at C-term. It is expected to recognise both reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Syntehtic peptide from the C-Terminus of the protein sequence according to NP_068556.2, NP_710141.1. Genename: FOXA2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 2000. Western blot: 0.5 μg/mL. Approx 60-65 kDa band observed in Human Liver lysate (predictedMW of 55 kDa according to NP_068556 and NP_710141).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FOXA2

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Alternative Name: FOXA2 / HNF3B (FOXA2 Products)

Synonyms: hnf3b antibody, tcf3b antibody, hnf3beta antibody, hnf3-beta antibody, FOXA2 antibody, HNF3B antibody, TCF3B antibody, HNF-3B antibody, ax antibody, axl antibody, fkd1 antibody, ik:tdsubc_2g8 antibody, tdsubc_2g8 antibody, wu:fe05g04 antibody, xx:tdsubc_2g8 antibody, FoxA2-A antibody, FoxA2a antibody, xFD-3 antibody, xbeta-1 antibody, HNF-3b antibody, HNF3-beta antibody, HNF3beta antibody, Hnf-3b antibody, Hnf3b antibody, Tcf-3b antibody, Tcf3b antibody, HNF-3BETA antibody, forkhead box A2 antibody, forkhead box A2 L homeolog antibody, foxa2 antibody, FOXA2 antibody, foxa2.L antibody, Foxa2 antibody

Background: FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.Synonyms: HNF-3-beta, HNF-3B, Hepatocyte nuclear factor 3-beta, TCF3B, Transcription factor 3B

Gene ID: 3170

NCBI Accession: NP_068556

UniProt: Q9Y261

Pathways: Dopaminergic Neurogenesis, Regulation of Carbohydrate Metabolic Process