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Zinc Finger, FYVE Domain Containing 20 (ZFYVE20) (C-Term) antibody

Details for Product No. ABIN374348, Supplier: Log in to see
Antigen
  • zfyve20
  • Rabenosyn-5
  • 5330426D11Rik
Epitope
C-Term
12
5
3
2
2
1
Reactivity
Human
27
11
3
3
2
2
Host
Goat
14
14
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Western Blotting (WB)
26
20
2
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Immunogen Peptide from C Terminus of the protein sequence according to NP_071735 Genename: ZFYVE20
Sequence C-ELKHTLAKQK GGTD
Specificity This antibody detects Rabenosyn-5 (ZFYVE20) at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name Rabenosyn-5 / ZFYVE20 (ZFYVE20 Antibody Abstract)
Background Synonyms: FYVE finger-containing Rab5 effector protein rabenosyn-5, Rabenosyn 5, Zinc finger FYVE domain-containing protein 20
Gene ID 64145
NCBI Accession NP_071735
UniProt Q9H1K0
Pathways
Application Notes Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.3 - 1.5 μg/mL. Approx 110 kDa band observed in A431, Fibroblast(epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-Zinc Finger, FYVE Domain Containing 20 (ZFYVE20) (C-Term) antibody (ABIN374348) anti-Zinc Finger, FYVE Domain Containing 20 (ZFYVE20) (C-Term) antibody
Background publications Nielsen, Christoforidis, Uttenweiler-Joseph et al.: "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain." in: The Journal of cell biology, Vol. 151, Issue 3, pp. 601-12, 2000 (PubMed).