ARHGEF4 antibody (N-Term)

Catalog No. ABIN374361

The Goat Polyclonal anti-ARHGEF4 antibody (ABIN374361) specifically detects ARHGEF4 in WB. The antibody is reactive with Human samples.

Quick Overview for ARHGEF4 antibody (N-Term) (ABIN374361)

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARHGEF4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARHGEF4 Antibody

Binding Specificity: N-Term

Sequence: PWEEPAGEKP SCS

Specificity: This antibody detects ARHGEF4 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_056135.2, NP_127462.1. Genename: ARHGEF4

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 2-4 μg/mL. Approx 80 kDa band observed in Human Brain lysates and intransfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.0 kDa accordingto NP_056135.2 and 76.0 kDa according to 127462.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for ARHGEF4

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Alternative Name: ARHGEF4

Background: ARHGEF4 may form complexes with G proteins and stimulate Rho-dependent signals, and is similar to rat collybistin protein. Rho GTPases play a fundamental role in numerous cellular processes, which are initiated by extracellular stimuli that work through G protein coupled receptors. Alternative splicing of this gene generates two transcript variants which encode different isoforms.Synonyms: APC-stimulated guanine nucleotide exchange factor, ASEF, KIAA1112, Rho guanine nucleotide exchange factor 4

Gene ID: 50649

NCBI Accession: NP_056135

UniProt: Q9NR80

Pathways: Neurotrophin Signaling Pathway