ARHGEF4 antibody (AA 143-271)

Catalog No. ABIN2749042

The Mouse Monoclonal anti-ARHGEF4 antibody (Clone ARHGEF-08) (ABIN2749042) specifically detects ARHGEF4 in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for ARHGEF4 antibody (AA 143-271) (ABIN2749042)

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARHGEF4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: ARHGEF-08

Product Details anti-ARHGEF4 Antibody

Binding Specificity: AA 143-271

Purpose: Anti-ARHGEF4 Purified

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant fragment of human ARHGEF4 (amino acids 143-271)

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for ARHGEF4

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Alternative Name: ARHGEF4

Background: Rho guanine nucleotide exchange factor 4,ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli –, stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.,ASEF1, GEF4, STM6

Gene ID: 50649

UniProt: Q9NR80

Pathways: Neurotrophin Signaling Pathway