ACAP1 antibody (C-Term)

Catalog No. ABIN374366

The Goat Polyclonal anti-ACAP1 antibody is suitable to detect ACAP1 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB.

Quick Overview for ACAP1 antibody (C-Term) (ABIN374366)

Target: ACAP1 (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 1 (ACAP1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ACAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ACAP1 Antibody

Binding Specificity: C-Term

Sequence: C-EKLSRRSHDL HTL

Specificity: This antibody detects ACAP1 / CENTB1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_055531.1 Genename: ACAP1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 80-90 kDa band observed in Jurkat lysates (predictedMW of 80 kDa according to NP_055531).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for ACAP1

Target: ACAP1 (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 1 (ACAP1))

Alternative Name: ACAP1 / CENTB1

Background: Potential GTPase-activating protein for the ADP ribosylation factor family, contains Contains 3 ANK repeats, 1 Arf GAP domain and 1 PH domain.Synonyms: ANK repeat and PH domain-containing protein 1, ARFGAP with coiled-coil, Centaurin beta 1, KIAA0050

Gene ID: 9744

NCBI Accession: NP_055531

UniProt: Q15027