AGAP3
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave an approx 45 kDa band in Human Brain andHuman Liver lysates at 1 μg/mL. This band was successfully blocked by incubation with theimmunising peptide. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Kidney shows strongstaining of brush borders in Proximal Convoluted tubules. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Target
AGAP3
(ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3))