MSX1 antibody (N-Term)

Catalog No. ABIN374442

Product Details anti-MSX1 Antibody

Target: MSX1 (Msh Homeobox 1 (MSX1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This MSX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: TSLPLGVKVE DS-C

Specificity: Recognizes MSX1 (N-term).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, and Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_002439.1.

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/16000. Western blot: Preliminary experiments gave an approx.55-60 kDa band in Human Muscleand Heart lysates at 1 μg/mL. Further, this band was successfully blocked byincubation with the immunising peptide. Please note that currently we cannot find anexplanation in the literature for the band we observe given the predicted size of approx.31.0 kDa according to Human NP_002439.2. Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Prostate shows staining of nucleoli in a few epithelial cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for MSX1

Target: MSX1 (Msh Homeobox 1 (MSX1))

Alternative Name: MSX1 (MSX1 Products)

Synonyms: ECTD3 antibody, HOX7 antibody, HYD1 antibody, STHAG1 antibody, AA675338 antibody, AI324650 antibody, Hox-7 antibody, Hox7 antibody, Hox7.1 antibody, msh antibody, CHOX-7 antibody, GHOX-7 antibody, HOX-7 antibody, msh homeobox 1 antibody, MSX1 antibody, Msx1 antibody

Background: MSX1 is a transcriptional repressor that belongs to the Msh homeobox family. It plays a role in cranofacial development, specifically in odontogenesis. A Sonic hedgehog-controlled cascade that includes MSX1 acts in parallel with the Wnt1 network to establish the midbrain dopaminergic progenitor domain, which gives rise to a neuronal population that is critical for normal function of the human brain and is involved in a number of neurological and psychiatric diseases, such as Parkinson's disease.Synonyms: HOX7, Homeobox protein Hox-7, Homeobox protein MSX-1, Hox-7, MSX-1, Msh homeobox 1-like protein

Gene ID: 4487, 9606

UniProt: P28360

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus