FHL1 antibody (Internal Region)

Catalog No. ABIN374471

The Goat Polyclonal anti-FHL1 antibody is suitable to detect FHL1 in samples from Human, Mouse, Rat, Dog, Cow and Pig. It has been validated for WB, IHC (p) and EIA.

Quick Overview for FHL1 antibody (Internal Region) (ABIN374471)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-FHL1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine, Porcine and Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001440.1, NP_001153171.1. Genename: FHL1

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.05-0.3 μg/mL. Detects a band of Approx 30 kDa in Human Muscle lysates(calculated MW of 31.0 kDa according to NP_001440). Immunohistochemistry: 2-2 μg/mL. In paraffin embedded Human Testis shows fibrousstaining in the cytoplasm of developing sperm cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: FHL1

Background: FHL1 (SLIM1) is a LIM domain protein transcript that is preferentially expressed in postnatal skeletal muscle but little is known about its function in this tissue. LIM proteins, (named for 'LIN11, ISL1 and MEC3') are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP J and plays a negative regulatory role in the RBP J mediated transcription in mammalian systems.Synonyms: FHL-1, FHL1, Four and a half LIM domains protein 1, SLIM, SLIM-1, SLIM1, Skeletal Muscle LIM-protein 1

Gene ID: 2273

NCBI Accession: NP_001153171

UniProt: Q13642