BID antibody (C-Term)

Catalog No. ABIN374543

The Goat Polyclonal anti-BID antibody is suitable to detect BID in samples from Human. It has been validated for WB and EIA.

Quick Overview for BID antibody (C-Term) (ABIN374543)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BID Antibody

Binding Specificity: C-Term

Sequence: C-TYVRSLARNG MD

Specificity: This antibody reacts to BH3-interacting domain death agonist. It is expected to recognise all three reported isoforms (as represented by NP_932070.1, NP_001187.1, NP_932071.1)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_932070.1, NP_001187.1, NP_932071.1. Genename: BID

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background: Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.Synonyms: BH3-interacting domain death agonist, p22 BID

Molecular Weight: 26.8kDa (NP_932070.1) and 22.0kDa (NP_001187.1).

Gene ID: 637, 9606

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity