PPY antibody (Internal Region)

Catalog No. ABIN374577

The Goat Polyclonal anti-PPY antibody (ABIN374577) specifically detects PPY in WB and IHC (p). The antibody is reactive with Human, Rat, Mouse and Dog samples.

Quick Overview for PPY antibody (Internal Region) (ABIN374577)

Target: PPY (Pancreatic Polypeptide (PPY))

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PPY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPY Antibody

Binding Specificity: Internal Region

Sequence: C-TRPRYGKRHK EDT

Specificity: This antibody recognizes Pancreatic prohormone.

Cross-Reactivity (Details): Species reactivity (expected):Rat and Canine (Dog).
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the internal region of the protein sequence according to NP_002713.1. Genename: PPY

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/32000. Western Blot: Preliminary experiments gave an approx 35-38 kDa band in human pancreaslysates after 1 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 10.8 kDaaccording to NP_002713.1. The 38 kDa band was successfully blocked by incubation withthe immunizing peptide. Immunohistochemistry: 3 μg/mL. In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cellslocated at the periphery of islets of Langerhans.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PPY

Target: PPY (Pancreatic Polypeptide (PPY))

Alternative Name: Pancreatic Prohormone

Background: Pancreatic hormone (PPY) is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.Synonyms: Obinepitide, PNP, PPY, Pancreatic polypeptide

Gene ID: 5539

NCBI Accession: NP_002713

UniProt: P01298

Pathways: Feeding Behaviour