CKB antibody (Internal Region)

Catalog No. ABIN374663

The Goat Polyclonal anti-CKB antibody is suitable to detect CKB in samples from Human, Rat, Mouse and Dog. It has been validated for WB.

Quick Overview for CKB antibody (Internal Region) (ABIN374663)

Target: CKB (Creatine Kinase, Brain (CKB))

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This CKB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CKB Antibody

Binding Specificity: Internal Region

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-KPSDEHKTDLNPDN, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/2000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CKB

Target: CKB (Creatine Kinase, Brain (CKB))

Alternative Name: CKBB

Background: Creatine kinase (CK) BB is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. Creatine kinase consists of two subunits, which can be either B (brain type) or M (muscle type). Therefore, three different cytosolic isoenzymes exist: CK-MM, CK-BB and CK-MB. Cytosolic CK isoenzymes are always co-expressed in a tissue specific fashion together with a mitochondrial isoform. MM is the major form in skeletal muscle and myocardium, MB exists in myocardium and BB in many tissues, especially brain.Synonyms: B-CK, CKB, Creatine kinase B chain, Creatine kinase B-type, Creatine kinase BB

NCBI Accession: NM_001823

UniProt: P12277