VANGL2 antibody (Internal Region)

Catalog No. ABIN374805

The Goat Polyclonal anti-VANGL2 antibody (ABIN374805) specifically detects VANGL2 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for VANGL2 antibody (Internal Region) (ABIN374805)

Target: VANGL2 (Vang-Like 2 (VANGL2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This VANGL2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VANGL2 Antibody

Binding Specificity: Internal Region

Sequence: C-QKYLRTTKQQ PYH

Specificity: This antibody detects Vang-like protein 2 (VANGL2).

Cross-Reactivity (Details): Species reactivity (expected):Cow, ZebrafishSpecies reactivity (tested):Human, Mouse

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_065068.1 Genename: VANGL2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.3-1 μg/mL. Approx 55 kDa band observed in Human Lung and Mouse Brainlysates (calculated MW of 59.5 kDa according to NP_065068.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for VANGL2

Target: VANGL2 (Vang-Like 2 (VANGL2))

Alternative Name: Vang-Like Protein 2 (VANGL2)

Background: VANGL2 is involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. It plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. It is required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process.Synonyms: KIAA1215, Loop-tail protein 1 homolog, STB1, Strabismus 1, Van Gogh-like protein 2

Gene ID: 57216, 9606

UniProt: Q9ULK5

Pathways: WNT Signaling, Stem Cell Maintenance, Tube Formation, Asymmetric Protein Localization