ASS1 antibody (Internal Region)

Catalog No. ABIN374809

Product Details anti-ASS1 Antibody

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ASS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequence: C-ENPKNQAPPG LYTKTQD

Specificity: This antibody detects ASS1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human, Cow.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000041.2, NP_446464.1. Genename: ASS1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: Approx 45 kDa band observed in Human Kidney lysates (calculated MW of46.5 kDa according to NP_000041.2 and NP_446464.1). Recommended concentration: 0.01-0.03 μg/mL. Immunofluorescence: A customer has reported this antibody works well in IF. Immunoprecipitation: This antibody has been successfully used in immunoprecipitationassays (Corbin et al. , 2008).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ASS1

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Alternative Name: ASS1 (ASS1 Products)

Synonyms: ASS antibody, CTLN1 antibody, AA408052 antibody, Ass-1 antibody, fold antibody, ASSA antibody, Ass antibody, ass antibody, zgc:92051 antibody, wu:fb95a04 antibody, wu:fc01e08 antibody, argininosuccinate synthase 1 antibody, argininosuccinate synthetase 1 antibody, argininosuccinate synthase 1 S homeolog antibody, ASS1 antibody, Ass1 antibody, ass1.S antibody, ass1 antibody

Background: ASS1 belongs to the argininosuccinate synthase family, type 1 subfamily. It catalyzes the penultimate step of the arginine biosynthetic pathway. Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700].Synonyms: Argininosuccinate synthase, Citrulline-aspartate ligase

Molecular Weight: 46.5 kDa (NP_000041.2 and NP_446464.1)

Gene ID: 445, 9606

UniProt: P00966

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin