DKC1 antibody (Internal Region)

Catalog No. ABIN374810

The Goat Polyclonal anti-DKC1 antibody has been validated for IHC (p). It is suitable to detect DKC1 in samples from Human and Mouse.

Quick Overview for DKC1 antibody (Internal Region) (ABIN374810)

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DKC1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DKC1 Antibody

Binding Specificity: Internal Region

Sequence: C-KRKRESESES DETPP

Specificity: This antibody detects Dyskerin.

Cross-Reactivity (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_001354.1 Genename: DKC1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 128000. Western blot: Preliminary experiments gave an approx 70-75 kDa band in Human BoneMarrow, Duodenum and Skin lysates after 0.01 μg/mL antibody staining. Please note thatcurrently we cannot find an explanation in the literature for the band we observe given thecalculated size of 57.5 kDa according to NP_001354.1. The 70-75 kDa band was successfullyblocked by incubation with the immunizing peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?Immunohistochemistry: In paraffin embedded Human Spleen shows strong nuclear

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for DKC1

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Alternative Name: DKC1 / NOLA4

Background: DKC1 (Dyskeratosis congenita 1) is a nucleolar protein, altered in dyskeratosis congenita, which carries out two separate functions, both fundamental for proliferating cells. One function is the pseudouridylation of ribosomal RNA (rRNA) molecules, necessary for their processing, and the other is the stabilization of the telomerase RNA component, necessary for telomerase activity. It is thought to be the catalytic subunit of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. DKC1 is also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Both 18S rRNA production and rRNA pseudouridylation are impaired if DKC1 is depleted.Synonyms: CBF5 homolog, Dyskerin, H/ACA ribonucleoprotein complex subunit 4, Nopp140-associated protein of 57 kDa, Nucleolar protein NAP57, Nucleolar protein family A member 4, snoRNP protein DKC1

Gene ID: 1736

NCBI Accession: NP_001354

UniProt: O60832

Pathways: Telomere Maintenance