GPSM2 antibody (Internal Region)

Catalog No. ABIN374868

Product Details anti-GPSM2 Antibody

Target: GPSM2 (G-Protein Signaling Modulator 2 (GPSM2))

Binding Specificity: Internal Region

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GPSM2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-NRLKGKKYKT NSSTK

Specificity: This antibody detects GPSM2.

Cross-Reactivity (Details): Species reactivity (expected):DogSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_037428.2 Genename: GPSM2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: 0.01-0.03 μg/mL. Approx 70 kDa band observed in Human Brain (Cerebellum)and Human Liver lysates (calculated MW of 75.0 kDa according to NP_037428.2). Anadditional band of unknown identity was also consistently observed at 26-20 kDa. Thisband was successfully blocked by incubation with the immunising peptide. We wouldappreciate any feedback from people in the field - have any such results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for GPSM2

Target: GPSM2 (G-Protein Signaling Modulator 2 (GPSM2))

Alternative Name: GPSM2 (GPSM2 Products)

Synonyms: CMCS antibody, DFNB82 antibody, LGN antibody, PINS antibody, dfnb82 antibody, lgn antibody, pins antibody, RGD1560967 antibody, Pinsa antibody, zgc:63574 antibody, 6230410J09Rik antibody, Pins antibody, G protein signaling modulator 2 antibody, G-protein signaling modulator 2 S homeolog antibody, G-protein signaling modulator 2 antibody, G-protein signalling modulator 2 (AGS3-like, C. elegans) antibody, GPSM2 antibody, gpsm2.S antibody, Gpsm2 antibody, gpsm2 antibody

Background: GPSM2 plays an important role in spindle pole orientation. It interacts and contributes to the functional activity of G(i) alpha proteins. GPSM2 acts to stabilize the apical complex during neuroblast divisions.Synonyms: G-protein-signaling modulator 2, GPSM-2, LGN, Mosaic protein LGN

Gene ID: 29899

NCBI Accession: NP_037428

UniProt: P81274

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling