MANF antibody (Internal Region)

Catalog No. ABIN374894

The Goat Polyclonal anti-MANF antibody is suitable to detect MANF in samples from Human, Mouse, Rat and Dog. It has been validated for WB.

Quick Overview for MANF antibody (Internal Region) (ABIN374894)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This MANF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MANF Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to ARMET.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence KFCREARGKENR, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: ARMET / ARP

Background: ARMET, also known as MANF, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, ARMET enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, ARMET has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of ARMET has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.Synonyms: Arginine-rich protein, MANF, Mesencephalic astrocyte-derived neurotrophic factor

Gene ID: 7873, 9606

UniProt: P55145