BHMT antibody (C-Term)

Catalog No. ABIN375080

The Goat Polyclonal anti-BHMT antibody is suitable to detect BHMT in samples from Human, Mouse, Rat and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for BHMT antibody (C-Term) (ABIN375080)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-BHMT Antibody

Binding Specificity: C-Term

Sequence: C-EQQLKELFEK QK

Specificity: This antibody recognizes BHMT.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence Genename: BHMT

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT

Background: BHMT (Betaine--homocysteine S-methyltransferase 1) is involved in the regulation of homocysteine metabolism. It is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. It is expressed exclusively in liver and kidney and displays differential expression in a model of liver cirrhosis.Synonyms: Betaine-homocysteine S-methyltransferase 1

Molecular Weight: 44.9kDa (Human NP_001704.1), 45.0kDa (Mouse NP_057877.1 and Rat NP_110477.1).

Gene ID: 635, 9606

UniProt: C6KNH7

Pathways: Methionine Biosynthetic Process