SFRP4 antibody (Internal Region)

Catalog No. ABIN375089

The Goat Polyclonal anti-SFRP4 antibody is suitable to detect SFRP4 in samples from Human, Rat, Mouse, Cow and Pig. It has been validated for WB, IHC (p) and EIA.

Quick Overview for SFRP4 antibody (Internal Region) (ABIN375089)

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This SFRP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SFRP4 Antibody

Binding Specificity: Internal Region

Specificity: This antibody recognizes SFRP4.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine and Porcine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TNSSCQCPHILPHQD, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: Detection limit dilution: 1/16000. Western blot: 0.3-1 μg/mL. Approx.40 kDa band observed in Human Uterus lysates(calculated MW of 39.0 kDa according to NP_003005.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 with 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SFRP4

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Alternative Name: SFRP4

Background: Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.Synonyms: FRP4, FRPHE, Frizzled protein human endometrium, SFRP-4, Secreted frizzled-related protein 4

Gene ID: 6424, 9606

UniProt: Q6FHJ7

Pathways: WNT Signaling