SFRP4 antibody (AA 22-303)

Catalog No. ABIN3044543

The Rabbit Polyclonal anti-SFRP4 antibody is suitable to detect SFRP4 in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for SFRP4 antibody (AA 22-303) (ABIN3044543)

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFRP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-SFRP4 Antibody

Binding Specificity: AA 22-303

Purpose: Anti-SFRP4 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human SFRP4 recombinant protein (Position: A22-K303). Human SFRP4 shares 96.8%,96.1% amino acid (aa) sequence identity with mouse,rat SFRP4,respectively.

Characteristics: Anti-SFRP4 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human SFRP4 recombinant protein (Position: A22-K303). Human SFRP4 shares 96.8% and 96.1% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -Western blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SFRP4

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Alternative Name: SFRP4

Background:

Background: Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. It is mapped to 7p14.1. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. And SFRP4 is a hub gene in a Type 2 Diabetes-associated gene coexpression module in human islets, and reduces glucose-induced insulin secretion through decreased β-cell exocytosis.

Gene Full Name: secreted frizzled related protein 4

Molecular Weight: 49 kDa

Gene ID: 6424

UniProt: Q6FHJ7

Pathways: WNT Signaling