VTCN1 antibody (Internal Region)

Catalog No. ABIN375113

The Goat Polyclonal anti-VTCN1 antibody is suitable to detect VTCN1 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB.

Quick Overview for VTCN1 antibody (Internal Region) (ABIN375113)

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This VTCN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VTCN1 Antibody

Binding Specificity: Internal Region

Sequence: C-SKGKGNANLE YK

Specificity: This antibody recognizes Human B7-H4.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_078902.2. Genename: VTCN1

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for VTCN1

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Alternative Name: VTCN1 / B7H4

Background: B7-H4 is a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected, suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response. B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC).Synonyms: B7-H4, B7S1, B7h.5, B7x, UNQ659/PRO1291, V-set domain-containing T-cell activation inhibitor 1

Gene ID: 79679, 9606

UniProt: Q7Z7D3