EGR2 antibody (Internal Region)

Catalog No. ABIN375194

The Goat Polyclonal anti-EGR2 antibody is suitable to detect EGR2 in samples from Human, Mouse, Rat and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for EGR2 antibody (Internal Region) (ABIN375194)

Target: EGR2 (Early Growth Response 2 (EGR2))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This EGR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-EGR2 Antibody

Binding Specificity: Internal Region

Sequence: HGTAGPDRKP FPC

Specificity: This antibody recognizes EGR2

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000390.2. Genename: EGR2

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.03-0.1 μg/mL. Approx 55 kDa band observed in lysates of cell line HepG2(calculated MW of 50.5 kDa according to NP_000390.2). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Colon shows nuclearstaining of absorptive cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EGR2

Target: EGR2 (Early Growth Response 2 (EGR2))

Alternative Name: EGR2

Background: ERG2 is a sequence-specific transcription factor, that binds to two specific DNA sites located in the promoter region of HOXA4. Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN), which can be inherited in either an autosomal dominant or recessive manner. Recessive CHN can present as Charcot-Marie-Tooth disease type 4E, type 1D, or Dejerine-Sottas syndrome (DSS), a group of neuropathies associated with decreased nerve conduction velocities.Synonyms: AT591, EGR-2, Early growth response protein 2, KROX20, Krox 20, Zinc finger protein Krox-20

Gene ID: 1959

NCBI Accession: NP_000390

UniProt: P11161