ICAM1 antibody

Catalog No. ABIN375487

This Rat Monoclonal antibody specifically detects ICAM1 in WB, FACS, IP, ICC, IHC (fro), BR and Purif. It exhibits reactivity toward Mouse and Pig.

Quick Overview for ICAM1 antibody (ABIN375487)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Mouse, Pig

Host: Rat

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Purification (Purif)

Clone: YN1-1-7-4

Product Details anti-ICAM1 Antibody

Purpose: Rat Anti-Mouse CD54-LE/AF

Specificity: Mouse/Porcine CD54, Mr 95 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD54 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, immunoprecipitation, blocking, and purification assays.

Endotoxin Level: Low Endotoxin

Immunogen: Mouse NS-1 cells

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54

Background: CD54, a member of the immunoglobulin superfamily of cell surface receptors and also known as Intercellular Adhesion Molecule-1 (ICAM-1), is a type I transmembrane glycoprotein that mediates important cell/cell interactions. CD54 is expressed on endothelial cells, dendritic cells, keratinocytes and lymphocytes. Its expression is upregulated by inflammatory mediators such as IFN- γ, IL-1, TNF- α, lipopolysaccharide and phorbol esters. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on antigen-presenting cells contributes to antigen-specific T-cell activation, presumably by enhancing interactions between T cell and antigen-presenting cells. These adhesion reactions are mediated via the binding of CD54 to its major ligands, LFA-1 and Mac-1.

Gene ID: 15894

UniProt: P13597

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling