Goat anti-Mouse IgG2a Antibody (HRP) - Preadsorbed

Catalog No. ABIN376250

Quick Overview for Goat anti-Mouse IgG2a Antibody (HRP) - Preadsorbed (ABIN376250)

Target: IgG2a

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISpot

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgG2a, Human ads-HRP

Specificity: Reacts with the heavy chain of mouse IgG 2a as demonstrated by ELISA and flow cytometry.

Characteristics: Goat Anti-Mouse IgG2a-HRP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.

Purification:

Purified by affinity chromatography on mouse IgG2a covalently linked to agarose, cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgG2a

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 % Glycerol/50 % Phosphate buffered saline, pH 7.4

Storage: 4 °C

Storage Comment: 2-8°C

References

Staszewski, Reece, ODonnell, Cunningham: "Drug treatment of malaria infections can reduce levels of protection transferred to offspring via maternal immunity." in: Proceedings. Biological sciences / The Royal Society, (2012) (PubMed).

Yu, Vajdy: "A novel retinoic acid, catechin hydrate and mustard oil-based emulsion for enhanced cytokine and antibody responses against multiple strains of HIV-1 following mucosal and systemic vaccinations." in: Vaccine, Vol. 29, Issue 13, pp. 2429-36, (2011) (PubMed).

Stegmann, Kamphuis, Meijerhof, Goud, de Haan, Wilschut: "Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation." in: Vaccine, (2010) (PubMed).

Target Details

Target: IgG2a

Target Type: Antibody

Background:

Description: Goat Anti-Mouse IgG2a, Human ads-HRP

NCBI_Official_Symbol: Ighg2a

Gene ID: 380793

UniProt: P01863