Goat anti-Mouse IgG Antibody (PE) - Preadsorbed

Catalog No. ABIN376440

Product Details

Target: IgG

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Specificity: Reacts with the heavy chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3

Characteristics: Goat Anti-Mouse IgG, Human ads-PE

Purification:

Purification Method: Affinity chromatography on mouse IgG covalently linked to agarose.

Preadsorption: Human Adsorbed

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB , Multiplex
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BGAL conjugate 1:500 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, CY5, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Wang, Hilchey, DeDiego, Perry, Hyrien, Nogales, Garigen, Amanat, Huertas, Krammer, Martinez-Sobrido, Topham, Treanor, Sangster, Zand: "Broad cross-reactive IgG responses elicited by adjuvanted vaccination with recombinant influenza hemagglutinin (rHA) in ferrets and mice." in: PLoS ONE, Vol. 13, Issue 4, pp. e0193680, (2018) (PubMed).

Broughton, Hercus, Nero, Kan, Barry, Dottore, Cheung Tung Shing, Morton, Dhagat, Hardy, Wilson, Downton, Schieber, Hughes, Lopez, Parker: "A dual role for the N-terminal domain of the IL-3 receptor in cell signalling." in: Nature communications, Vol. 9, Issue 1, pp. 386, (2018) (PubMed).

Kristian, Ota, Bubeck, Cho, Groff, Kubota, Destito, Martin, Laudenslager, Koriazova, Tahara, Kanda: "Generation and Improvement of Effector Function of a Novel Broadly Reactive and Protective Monoclonal Antibody against Pneumococcal Surface Protein A of Streptococcus pneumoniae." in: PLoS ONE, Vol. 11, Issue 5, pp. e0154616, (2017) (PubMed).

Geno, Spencer, Nahm: "Rapid and efficient purification of ficolin-2 using a disposable CELLine bioreactor." in: Journal of immunological methods, Vol. 424, pp. 106-10, (2015) (PubMed).

Warram, Sorace, Saini, Borovjagin, Hoyt, Zinn: "Systemic delivery of a breast cancer-detecting adenovirus using targeted microbubbles." in: Cancer gene therapy, Vol. 19, Issue 8, pp. 545-52, (2013) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody