HA-Tag antibody (HRP)

Details for Product No. ABIN387712, Supplier: Log in to see
Antigen
Reactivity
Tag
450
2
1
1
1
1
1
Host
Mouse
180
177
40
34
20
3
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
42
29
23
8
8
7
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
360
200
115
109
81
70
53
40
15
13
11
7
7
5
4
4
4
4
3
2
2
1
1
1
1
1
1
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Brand THE™
Immunogen A synthetic peptide (YPYDVPDYA) coupled - KLH
Sequence YPYDVPDYA
Clone 5E11D8
Isotype IgG
Specificity THETM HA Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins.
Characteristics The antibody is suitable for detecting the expression level of HA in fusion proteins. Do not add azide as it inhibits HRP activity.
Purification Protein A affinity column
Alternative Name HA Tag
Target Type Tag
Research Area Tags/Labels
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-0.5 µg/mL
Western blot: 0.1-0.5 µg/mL
Other applications: user-optimized
Restrictions For Research Use only
Format Lyophilized
Concentration 0.5 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Supplier Images
 image for anti-HA-Tag antibody (HRP) (ABIN387712) Lane 1-3: 2 µL, 0.4 µL, 0.08 µL N-terminal HA-GST fusion protein in E. coli cell lysa...
 image for anti-HA-Tag antibody (HRP) (ABIN387712) 293 cells transfected with N-terminal HA tag protein. Primary antibody: 1 µg/mL Anti-...
 image for anti-HA-Tag antibody (HRP) (ABIN387712) 293 cells transfected with C-terminal HA tag protein. Primary antibody: 1 µg/mL Anti-...
Product cited in: Haac, Anderson, Eggleston, Myles, Adelman: "The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes." in: Nucleic acids research, Vol. 43, Issue 7, pp. 3688-700, 2015 (PubMed).