HIS Tag antibody (FITC)
| Antigen |
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| Clonality |
Monoclonal (6G2A9) |
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Host
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Conjugate
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HRP (12), Biotin (10), FITC (7), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alkaline Phosphatase (AP) (2), HiLyte Fluor 488 (2), HiLyte Fluor 555 (2), HiLyte Fluor 647 (2), PE,Cy5.5 (2), AMCA (1), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FAM (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1), ROX (1), Sepharose (1), TAMRA (1)
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Application
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Western Blotting (WB) (109), ELISA (73), Immunoprecipitation (IP) (48), Immunofluorescence (IF) (45), Flow Cytometry (FACS) (31), Immunocytochemistry (ICC) (12), Immunohistochemistry (IHC) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Dot Blot (Dot) (4), Transfected cell culture (TCC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
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| Catalog no. |
ABIN387722 |
| Quantity |
100ug (1 mg/ml, lyophilized with PBS, pH 7...) |
| Price |
410.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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His-tag
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Immunogen
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A synthetic peptide HHHHHH coupled to KLH
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Format
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Purified
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Isotype
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IgG1, kappa chain
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Clone
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6G2A9
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Description
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Antibodies-Online Anti-His-tag [FITC] Monoclonal Antibody is an FITC conjugated Anti-His Monoclonal Antibody (Mouse) (ABIN387699) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
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Specificity
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Antibodies-Online Anti-His-tag [FITC] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
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Application Notes
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Immunofluorescence (IF): 0.1-2 ug/mlFlow cytometry: 0.1-2 ug for 1 x 106 cellsOther applications: User-optimized
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Concentration
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1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
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Purification
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Protein A affinity column
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Storage
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GenScript Anti-His-tag [FITC] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "HIS Tag", type "Antibodies"
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Hosts
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Mouse (77), Rabbit (54), Chicken (11), Goat (7), Sheep (1)
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Reactivities
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Fusion Protein (Tag) (14), Human (11), All Species (9), Mouse (Murine) (8), Rat (Rattus) (6), Tag (4), Cow (Bovine) (1), Dog (Canine) (1), Pig (Porcine) (1), Rabbit (1), Various Species (1)
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Applications
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Western Blotting (WB) (109), ELISA (73), Immunoprecipitation (IP) (48), Immunofluorescence (IF) (45), Flow Cytometry (FACS) (31), Immunocytochemistry (ICC) (12), Immunohistochemistry (IHC) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Dot Blot (Dot) (4), Transfected cell culture (TCC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
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Conjugates
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HRP (12), Biotin (10), FITC (7), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alkaline Phosphatase (AP) (2), HiLyte Fluor 488 (2), HiLyte Fluor 555 (2), HiLyte Fluor 647 (2), PE,Cy5.5 (2), AMCA (1), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FAM (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1), ROX (1), Sepharose (1), TAMRA (1)
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Alternatives 
