DNMT3A antibody (AA 457-486)

Catalog No. ABIN387882

Cited in 2+ publications. The Rabbit Polyclonal anti-DNMT3A antibody (Clone RB15269) (ABIN387882) specifically detects DNMT3A in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for DNMT3A antibody (AA 457-486) (ABIN387882)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB15269

Product Details anti-DNMT3A Antibody

Binding Specificity: AA 457-486

Predicted Reactivity: C, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from the Central region of human Dnmt3a.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DNMT3A antibody (ABIN387882)

Husni, Shiba-Ishii, Iiyama, Shiozawa, Kim, Nakagawa, Sato, Kano, Minami, Noguchi: "DNMT3a expression pattern and its prognostic value in lung adenocarcinoma." in: Lung cancer (Amsterdam, Netherlands), Vol. 97, pp. 59-65, (2017) (PubMed).

Chestnut, Chang, Price, Lesuisse, Wong, Martin: "Epigenetic regulation of motor neuron cell death through DNA methylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 46, pp. 16619-36, (2011) (PubMed).

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: Dnmt3a

Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

Molecular Weight: 101858

Gene ID: 1788

NCBI Accession: NP_072046, NP_715640, NP_783328, NP_783329

UniProt: Q9Y6K1