DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) antibody

Antigen: DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1)

Synonyms: ecat7, D6Ertd14e, MGC102308, DNMT3L

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387907

Additional Information

Alternative name: Dnmt3L

Gene ID: 54427

UniProt: Q9CWR8

Immunogen: This Mouse Dnmt3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392~421 amino acids from the mouse Dnmt3L sequence within the last 100 aa. The antibody may recognize the human homolog (44 k

Isotype: Ig

Description: Other names: DNA (cytosine-5)-methyltransferase 3-like

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Mouse Dnmt3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392~421 amino acids from the mouse Dnmt3L sequence within the last 100 aa. The antibody may recognize the human homolog (44 kDa).

Molecular Weight: 47992 DA

Comments:

Background: mDnmt3L (DNA methyltransferase 3L) does not appear to be catalytically active due to loss of the critical active site residues. It may function not directly as a DNA methyltransferase but rather as a regulator of methylation at imprinted loci. It is required specifically for the establishment of genomic imprints but is dispensable for their propagation. It is essential for the de novo methylation of single-copy DNA sequences. The protein is well-express in testis, thymus, ovary, and heart. mDnmt3L, which belongs to the 5-cytosine methyltransferase family, contains 1 ADD-type zinc finger.

Application Details

Application Notes: ELISA~~1:1,000 Western Blot ~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of Mouse Dnmt3L Antibody (ABIN387907) in Hela cell line lysates (35ug/lane). Dnmt3l (arrow) was detected using the purified Pab.

Publications

Product: Bourchis, Xu, Lin et al.: "Dnmt3L and the establishment of maternal genomic imprints." in: Science (New York, N.Y.), Vol. 294, Issue 5551, pp. 2536-9, 2001 (PubMed).

Okazaki, Furuno, Kasukawa et al.: "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs." in: Nature, Vol. 420, Issue 6915, pp. 563-73, 2002 (PubMed).