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Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (C-Term) antibody
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423~452 amino acids from the C-terminal region of human UBE1C.|
|Description||Other names: NEDD8-activating enzyme E1 catalytic subunit, Ubiquitin-activating enzyme 3, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, UBA3|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423~452 amino acids from the C-terminal region of human UBE1C.|
|Molecular Weight||51719 DA|
Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
Osaka, Kawasaki, Aida et al.: "A new NEDD8-ligating system for cullin-4A." in: Genes & development, Vol. 12, Issue 15, pp. 2263-8, 1998 (PubMed).
Gong, Yeh: "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 12036-42, 1999 (PubMed).
Gubin, Njoroge, Bouffard et al.: "Gene expression in proliferating human erythroid cells." in: Genomics, Vol. 59, Issue 2, pp. 168-77, 1999 (PubMed).
Walden, Podgorski, Schulman: "Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8." in: Nature, Vol. 422, Issue 6929, pp. 330-4, 2003 (PubMed).
Bohnsack, Haas: "Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26823-30, 2003 (PubMed).
|Reactivities||Human (9), Mouse (Murine) (2), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Rat (Rattus) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (8), ELISA (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (IHC) (1)|
|Epitopes||N-Term (4), C-Term (2)|