Histone Acetyltransferase 1 (HAT1) (N-Term) antibody

Antigen: Histone Acetyltransferase 1 (HAT1)

Synonyms: KAT1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387931

Additional Information

Alternative name: KAT1 (HAT1)

Gene ID: 8520

UniProt: O14929

Immunogen: This KAT1 (HAT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35~65 amino acids from the N-terminal region of human HAT1.

Isotype: Ig

Description: Other names: Histone acetyltransferase type B catalytic subunit, KAT1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KAT1 (HAT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35~65 amino acids from the N-terminal region of human HAT1.

Molecular Weight: 49513 DA

Comments:

Background: Histone acetylation, particularly of histone H4, has been proposed to play an important role in replication-dependent nucleosome assembly. The HAT1 protein contains D, A, and B motifs, which are present in many N-acetyltransferases, including those that acetylate substrates other than histones. The HAT1 holoenzyme consists of 2 subunits: the catalytic 46-kD HAT1 and the accessory p46. The p46 subunit stimulated the activity of HAT1 and bound to core histones. The HAT1 holoenzyme acetylated newly synthesized but not nucleosomal histone H4 at lys5 and lys12, and, to a lesser extent, histone H2A at lys5. HAT1 and p46 polypeptides are located in the nucleus of S-phase cells. HAT1 may play a role in telomeric silencing.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blot~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Signaling, Protein Modifications, Cancer, Cell Cycle, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of KAT1 (HAT1) Antibody (N-term) (ABIN387931) in HL60 cell line lysates (35ug/lane). HAT1 (arrow) was detected using the purified Pab.

Publications

Product: Cheung, Tanner, Cheung et al.: "Synergistic coupling of histone H3 phosphorylation and acetylation in response to epidermal growth factor stimulation." in: Molecular cell, Vol. 5, Issue 6, pp. 905-15, 2000 (PubMed).

Makowski, Dutnall, Annunziato: "Effects of acetylation of histone H4 at lysines 8 and 16 on activity of the Hat1 histone acetyltransferase." in: The Journal of biological chemistry, Vol. 276, Issue 47, pp. 43499-502, 2001 (PubMed).

Grönroos, Hellman, Heldin et al.: "Control of Smad7 stability by competition between acetylation and ubiquitination." in: Molecular cell, Vol. 10, Issue 3, pp. 483-93, 2002 (PubMed).