Lysine (K)-Specific Demethylase 3A (KDM3A) (N-Term) antibody

Antigen: Lysine (K)-Specific Demethylase 3A (KDM3A)

Synonyms: tsga, jmjd1, jhdm2a, jhmd2a, jmjd1a, TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, DKFZp686A24246, DKFZp686P07111, TGSA, Tsga, Jmjd1, KDM2A, JHDM2a, Jmjd1a, mKIAA0742, 1700105C21Rik, C230043E16Rik, KDM3A

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN387995

Additional Information

Alternative name: JHDM2a

Gene ID: 55818

UniProt: NP_060903

Immunogen: This JHDM2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57~86 amino acids from the N-terminal region of human JHDM2A.

Isotype: Ig

Description: Other names: TSGA, JMJD1, KDM3A, JHDM2A, JHMD2A, KIAA0742, DKFZp686A24246, DKFZp686P07111, JMJD1A

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This JHDM2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57~86 amino acids from the N-terminal region of human JHDM2A.

Molecular Weight: 147211 DA

Comments:

Background: JHDM2a is a zinc finger protein that contains a jumonji domain. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. This protein preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. It is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation.

Synonyms: tsga, jmjd1, jhdm2a, jhmd2a, jmjd1a, TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, DKFZp686A24246, DKFZp686P07111, TGSA, Tsga, Jmjd1, KDM2A, JHDM2a, Jmjd1a, mKIAA0742, 1700105C21Rik, C230043E16Rik, KDM3A

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Protein Modifications

Restrictions: For Research Use only

Images

JHDM2a Antibody (N-term) (ABIN387995) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the JHDM2a antibody detected the JHDM2a protein (arrow).

JHDM2a Antibody (N-term) (ABIN387995)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JHDM2a Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Yamane, Toumazou, Tsukada et al.: "JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor." in: Cell, Vol. 125, Issue 3, pp. 483-95, 2006 (PubMed).

Wellmann, Bettkober, Zelmer et al.: "Hypoxia upregulates the histone demethylase JMJD1A via HIF-1." in: Biochemical and biophysical research communications, Vol. 372, Issue 4, pp. 892-7, 2008 (PubMed).