SUMO2/3 antibody (C-Term)

Catalog No. ABIN388034

There are 8+ publications for this product available. The Rabbit Polyclonal anti-SUMO2/3 antibody is suitable to detect SUMO2/3 in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for SUMO2/3 antibody (C-Term) (ABIN388034)

Target: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUMO2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB00635

Product Details anti-SUMO2/3 Antibody

Binding Specificity: AA 49-81, C-Term

Predicted Reactivity: X, Zf, B, C, Ha, Pr, Pig

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-81 amino acids from the C-terminal region of human SUMO2/3.

Isotype: IgG

Application Details

Application Notes: IF: 1:100. IF: 1:100. WB: 1:2000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-SUMO2/3 antibody (ABIN388034)

Higginbotham, OShea: "Adenovirus E4-ORF3 Targets PIAS3 and Together with E1B-55K Remodels SUMO Interactions in the Nucleus and at Virus Genome Replication Domains." in: Journal of virology, Vol. 89, Issue 20, pp. 10260-72, (2015) (PubMed).

Myles, Fontecilla, Valdez, Vithayathil, Naik, Belkaid, Ouyang, Datta: "Signaling via the IL-20 receptor inhibits cutaneous production of IL-1β and IL-17A to promote infection with methicillin-resistant Staphylococcus aureus." in: Nature immunology, Vol. 14, Issue 8, pp. 804-11, (2013) (PubMed).

Heo, Lee, Nigro, Thomas, Le, Chang, McClain, Reinhart-King, King, Berk, Fujiwara, Woo, Abe: "PKC? mediates disturbed flow-induced endothelial apoptosis via p53 SUMOylation." in: The Journal of cell biology, Vol. 193, Issue 5, pp. 867-84, (2011) (PubMed).

Nadtochiy, Redman, Rahman, Brookes: "Lysine deacetylation in ischaemic preconditioning: the role of SIRT1." in: Cardiovascular research, Vol. 89, Issue 3, pp. 643-9, (2011) (PubMed).

Snider, Weerasinghe, Iñiguez-Lluhí, Herrmann, Omary: "Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation." in: The Journal of biological chemistry, Vol. 286, Issue 3, pp. 2273-84, (2011) (PubMed).

Cho, Yi, Tserentsoodol, Searle, Ferreira: "Neuroprotection resulting from insufficiency of RANBP2 is associated with the modulation of protein and lipid homeostasis of functionally diverse but linked pathways in response to oxidative stress." in: Disease models & mechanisms, Vol. 3, Issue 9-10, pp. 595-604, (2010) (PubMed).

Martin, Schwamborn, Urlaub, Gan, Guan, Dejean: "Spatial interplay between PIASy and FIP200 in the regulation of signal transduction and transcriptional activity." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2771-81, (2008) (PubMed).

Degerny, Monte, Beaudoin, Jaffray, Portois, Hay, de Launoit, Baert: "SUMO modification of the Ets-related transcription factor ERM inhibits its transcriptional activity." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 24330-8, (2005) (PubMed).

Target Details for SUMO2/3

Target: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Alternative Name: SUMO2/3

Background: SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

Molecular Weight: 11637

Gene ID: 6612

NCBI Accession: NP_008867

UniProt: P55854