SMT3 Suppressor of Mif Two 3 Homolog 2/3 (SUMO2/3) (C-Term) antibody

Antigen: SMT3 Suppressor of Mif Two 3 Homolog 2/3 (SUMO2/3)

Synonyms: HSMT3, SMT3B, SMT3H2, MGC117191, zgc:92241, SUMO2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388034

Additional Information

Alternative name: SUMO2/3

Gene ID: 6612

UniProt: P55854

Immunogen: This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids within C-terminal region of human SUMO2/3.

Isotype: Ig

Description: Other names: Small ubiquitin-like modifier, Ubiquitin-like protein SMT3B, Sentrin 2, HSMT3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids within C-terminal region of human SUMO2/3.

Molecular Weight: 11637 DA

Comments:

Background: SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Apoptosis/Necrosis, Translation Factors, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

The anti-SUMO2/3 C-term Pab (ABIN388034) is used in Western blot to detect SUMO2/3 in HeLa cell lysate.

COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with ABIN388027 SUMO-1 antibody. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with ABIN388034 SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Lapenta, Chiurazzi, van der Spek et al.: "SMT3A, a human homologue of the S. cerevisiae SMT3 gene, maps to chromosome 21qter and defines a novel gene family." in: Genomics, Vol. 40, Issue 2, pp. 362-6, 1997 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).