SUMO2/3 antibody

Catalog No. ABIN6945095

The Rabbit Monoclonal anti-SUMO2/3 antibody (Clone 7G10) (ABIN6945095) specifically detects SUMO2/3 in WB, IHC, IHC (p) and IF (cc). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for SUMO2/3 antibody (ABIN6945095)

Target: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SUMO2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 7G10

Product Details anti-SUMO2/3 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: C terminal Human Sumo 2+3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SUMO2/3

Target: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Alternative Name: Sumo 2+3

Background:

Synonyms: Small ubiquitin-related modifier 2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Small ubiquitin-related modifier 3, SMT3 homolog 1, SUMO-2, Ubiquitin-like protein SMT3A, SUMO-2, Smt3B, SUMO2, SUMO-3, Smt3A, SUMO3

Background: The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease.

UniProt: P55854, P61956