Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (N-Term) antibody

Antigen: Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL)

Synonyms: cbl, Cbl-2, c-Cbl, 4732447J05Rik, CBL2, C-CBL, RNF55

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388071

Additional Information

Alternative name: Cbl

Gene ID: 23624

UniProt: Q9ULV8

Immunogen: This CBLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids from the N-terminal region of human Cbl-c.

Isotype: Ig

Description: Other names: Signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, RING finger protein 57, RNF57, CBLC

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CBLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids from the N-terminal region of human Cbl-c.

Molecular Weight: 52456 DA

Comments:

Background: CBLC proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl- c is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Through interactions with proteins containing SRC homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

CBLC Antibody (R10) (ABIN388071) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CBLC antibody detected the CBLC protein (arrow).

Publications

Product: Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).