Bone Morphogenetic Protein 6 (BMP6) (N-Term) antibody

Antigen: Bone Morphogenetic Protein 6 (BMP6)

Synonyms: VGR, VGR1, Vgr1, D13Wsu115e, BMP6, zgc:113595, vgr, vgr1, vgr-1, MGC115308

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388456

Additional Information

Alternative name: Bmp6

Gene ID: 654

UniProt: NP_001709

Immunogen: This Bmp6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33~62 amino acids from the N-terminal region of human Bmp6.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Bmp6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33~62 amino acids from the N-terminal region of human Bmp6.

Molecular Weight: 57225 DA

Comments:

Background: The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, Bmp6 has a proposed role in early development. In addition, the fact that BMP6 is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

The anti-Bmp6 (N-term) Pab (ABIN388456) is used in Western blot to detect Bmp6 in HL60 cell lysate.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bmp6 antibody (N-term)(ABIN388456), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Olavesen, Bentley, Mason et al.: "Fine mapping of 39 ESTs on human chromosome 6p23-p25." in: Genomics, Vol. 46, Issue 2, pp. 303-6, 1998 (PubMed).

Tamada, Kitazawa, Gohji et al.: "Molecular cloning and analysis of the 5'-flanking region of the human bone morphogenetic protein-6 (BMP-6)." in: Biochimica et biophysica acta, Vol. 1395, Issue 3, pp. 247-51, 1998 (PubMed).

Bobacz, Gruber, Soleiman et al.: "Expression of bone morphogenetic protein 6 in healthy and osteoarthritic human articular chondrocytes and stimulation of matrix synthesis in vitro." in: Arthritis and rheumatism, Vol. 48, Issue 9, pp. 2501-8, 2003 (PubMed).

Lories, Derese, Ceuppens et al.: "Bone morphogenetic proteins 2 and 6, expressed in arthritic synovium, are regulated by proinflammatory cytokines and differentially modulate fibroblast-like synoviocyte apoptosis." in: Arthritis and rheumatism, Vol. 48, Issue 10, pp. 2807-18, 2003 (PubMed).