LC3 (APG8C) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN388478
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Antigen
Epitope
N-Term, AA 1-30
(1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(1)
Host
Rabbit
(1)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN388478
291.50 $
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Immunogen This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human LC3.
Clone RB7613
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3c is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Synonyms: MAP1LC3C, microtubule-associated protein 1 light chain 3 gamma, LC3C, MAP1LC3C
Molecular Weight 16852 Da
Gene ID 440738
UniProt Q9BXW4
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes Suggested dilutions:
WB = 1:1000, IHC = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-LC3 (APG8C) (N-Term), (AA 1-30) antibody The anti-LC3 (APG8c) Pab (ABIN388478) is used in Western blot to detect LC3 (APG8c) in, from left to right, 293, Ramos, Hela, HepG2, T47d, and Jurkat tissue lysates. LC3 (APG8c)(arrow) was detected using the purified polyclonal antibody.
anti-LC3 (APG8C) (N-Term), (AA 1-30) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

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