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Details for Product No. ABIN388483

Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 89-120), (cleaved), (C-Term) antibody

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Antigen
Synonyms ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, LC3a, 1010001H21Rik, 4922501H04Rik, zgc:77094, MGC69006, map1lc3a, MGC53333, MGC89867, MAP1LC3A
Epitope
»Alternatives AA 89-120, cleaved, C-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality (Clone) Polyclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Pubmed 6 references available
Catalog no. ABIN388483
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 AA from human Cleaved LC3A.
Clone RB38908
Isotype Ig
Specificity This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~120 amino acids from the C-terminal cleavage site of human cleaved-LC3 (APG8a).
Predicted Reactivity Cow (Bovine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name LC3A
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate
Molecular Weight 14272 Da
Gene ID 84557
UniProt Q9H492
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes Recommend dilutions: IF : 1:100,WB : 1:1000,IHC : 1:10-50,ICC : 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Sivridis, Koukourakis, Zois et al.: "LC3A-positive light microscopy detected patterns of autophagy and prognosis in operable breast carcinomas." in: The American journal of pathology, Vol. 176, Issue 5, pp. 2477-89, 2010 (PubMed).

Alternatives for antigen "Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A)", type "Antibodies"
Hosts (114), (10), (5), (3)
Reactivities (129), (92), (83), (49), (26), (25), (16), (12), (9), (3), (3), (2), (2), (1), (1), (1), (1)
Applications (97), (43), (32), (31), (30), (30), (15), (13), (6), (5), (3), (2), (2), (1)
Conjugates (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Epitopes (24), (15), (6), (5), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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