Did you know that you can buy products from over 140 different suppliers from us?

ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B) (AA 258-284) antibody

Details for Product No. ABIN388497
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms apg4b, APG4B, AUTL1, 2510009N07Rik, AW048066, Apg4b, Atg4bl, Autl1, Aut2b2
Epitope
AA 258-284
(15), (12), (9), (8), (7), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(89), (35), (17), (15), (14), (14), (2), (2), (2), (1), (1)
Host
Rabbit
(86), (3), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(68), (30), (27), (20), (15), (2), (2), (2), (1)
Pubmed 2 references available
Quantity 400 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN388497
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-284 AA from human ATG4B.
Clone RB11843
Isotype Ig
Specificity This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264~293 amino acids from the central region of human APG4B.
Predicted Reactivity Cow (Bovine),Chicken,Mouse (Murine),Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATG4B
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4, a cysteine protease required for autophagy, cleaves the C-terminal part of MAP1LC3 to form the activated molecule LC3-I. LC3-I is subsequently conjugated with phosphatidylethanolamine at the C-terminal glycine to form LC3-II, a marker for autophagy via its capacity to bind to autophagosomes.
Synonyms: APG4B, AUTL1, MGC1353, APG4 autophagy 4 homolog B, AUT-like 1 cysteine endopeptidase, autophagin-1, autophagy-related cysteine endopeptidase 1, autophagy-related protein 4 homolog B, cysteine protease ATG4B, hAPG4B
Molecular Weight 44309 DA
Gene ID 23192
UniProt Q9Y4P1
Research Area Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B) (AA 258-284) antibody Western blot analysis of APG4B (arrow) using purified polyclonal antibody (ABIN388497). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the APG4B gene (Lane 2) (Origene Technologies).
anti-ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B) (AA 258-284) antibody (2) Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Autophagy APG4B Antibody (E273)(ABIN388497), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Mariño, Uría, Puente et al.: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).

Tanida, Sou, Ezaki et al.: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).

Validation Images
back to top