ATG4B antibody (AA 258-284)

Catalog No. ABIN388497

The Rabbit Polyclonal anti-ATG4B antibody (Clone RB11843) (ABIN388497) specifically detects ATG4B in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for ATG4B antibody (AA 258-284) (ABIN388497)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB11843

Product Details anti-ATG4B Antibody

Binding Specificity: AA 258-284

Predicted Reactivity: B, C, Zf, M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-284 amino acids from human ATG4B.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: ATG4B

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4, a cysteine protease required for autophagy, cleaves the C-terminal part of MAP1LC3 to form the activated molecule LC3-I. LC3-I is subsequently conjugated with phosphatidylethanolamine at the C-terminal glycine to form LC3-II, a marker for autophagy via its capacity to bind to autophagosomes.

Molecular Weight: 44294

Gene ID: 23192

NCBI Accession: NP_037457, NP_847896

UniProt: Q9Y4P1

Pathways: Autophagy