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ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 494-523) antibody

Details for Product No. ABIN388522
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Antigen
Synonyms 1810013K23Rik, Agp7, Apg7l, Atg7l, APG7-LIKE, APG7L, GSA7, ATAPG7, ATATG7, ATG7, AUTOPHAGY 7, AUTOPHAGY-RELATED 7, K15I22.10, K15I22_10, AO090001000744, atg7, UBE1, UBE1L, UBE1L2
Epitope
AA 494-523
(14), (12), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(58), (22), (13), (1), (1)
Host
Rabbit
(57), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
(44), (18), (16), (14), (13), (10), (8), (3), (3), (1), (1), (1), (1)
Pubmed 7 references available
Quantity 400 µL
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Catalog No. ABIN388522
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 AA from human ATG7.
Clone RB7472
Isotype Ig
Specificity This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 501~531 amino acids surrounding amino acid R509 of human APG7L.
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name ATG7
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
Synonyms: APG7-LIKE, APG7L, DKFZp434N0735, GSA7, APG7 autophagy 7-like, autophagy-related protein 7, hAGP7, ubiquitin activating enzyme E1-like protein, ubiquitin-activating enzyme E1-like protein
Pathways: Regulation of autophagy
Molecular Weight 77960 Da
Gene ID 10533
UniProt O95352
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes IF = 1:200, WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 494-523) antibody APG7L Antibody (R509) (ABIN388522) western blot analysis in Hela cell line lysates (35 µg/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).
anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 494-523) antibody (2) Western blot analysis of anti-hAPG7L-R509 Pab (ABIN388522) in 293 cell line lysates transiently transfected with the ATG7 gene (2 µg/lane). hAPG7L-R509(arrow) was detected using the purified polyclonal antibody (1:60 dilution).
anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 494-523) antibody (3) The anti-APG7L Pab (ABIN388522) is used in Western blot to detect APG7L in mouse liver tissue lysate. APG7L(arrow) was detected using the purified polyclonal antibody.
Background publications Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Tanida, Tanida-Miyake, Nishitani et al.: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN388522
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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