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ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 494-523) antibody

Details for Product No. ABIN388522, Supplier: Log in to see
Antigen
  • 1810013K23Rik
  • Agp7
  • AO090001000744
  • APG7-LIKE
  • Apg7l
  • APG7L
  • ATAPG7
  • ATATG7
  • atg7
  • ATG7
  • Atg7l
  • AUTOPHAGY-RELATED 7
  • AUTOPHAGY 7
  • GSA7
  • K15I22.10
  • K15I22_10
Epitope
AA 494-523
30
19
15
14
13
8
7
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
149
44
26
Host
Rabbit
143
10
3
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
4
4
4
4
4
4
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
120
63
57
35
22
16
12
6
6
3
3
2
1
Supplier
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Immunogen This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 AA from human ATG7.
Clone RB7472
Isotype Ig
Specificity This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 501~531 amino acids surrounding amino acid R509 of human APG7L.
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name ATG7 (ATG7 Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
Synonyms: APG7-LIKE, APG7L, DKFZp434N0735, GSA7, APG7 autophagy 7-like, autophagy-related protein 7, hAGP7, ubiquitin activating enzyme E1-like protein, ubiquitin-activating enzyme E1-like protein
Pathways: Regulation of autophagy
Molecular Weight 77960 Da
Gene ID 10533
UniProt O95352
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes IF = 1:200, WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 494-523) antibody (ABIN388522) APG7L Antibody (R509) (ABIN388522) western blot analysis in Hela cell line lysates (3...
Western Blotting (WB) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 494-523) antibody (ABIN388522) Western blot analysis of anti-hAPG7L-R509 Pab (ABIN388522) in 293 cell line lysates t...
Western Blotting (WB) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 494-523) antibody (ABIN388522) The anti-APG7L Pab (ABIN388522) is used in Western blot to detect APG7L in mouse live...
Product cited in: Okamoto, Sakimoto, Imai, Senoo, Shidoji: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, 2011

Wei, Kulp, Chen: "Energy restriction as an antitumor target of thiazolidinediones." in: The Journal of biological chemistry, Vol. 285, Issue 13, pp. 9780-91, 2010

Yang, Liu, Goga, Kim, Yuneva, Bishop: "Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 31, pp. 13836-41, 2010

Bigford, Alonso, Dietrich, Keane: "A novel protein complex in membrane rafts linking the NR2B glutamate receptor and autophagy is disrupted following traumatic brain injury." in: Journal of neurotrauma, Vol. 26, Issue 5, pp. 703-20, 2009

Ding, Shi, Zhou, Qiu, Xu, Dai, Shi, Wang, Ke, Wu, Fan: "Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma." in: Cancer research, Vol. 68, Issue 22, pp. 9167-75, 2008

Xu, Liang, Haridas, Gaikwad, Connolly, Mills, Gutterman: "A plant triterpenoid, avicin D, induces autophagy by activation of AMP-activated protein kinase." in: Cell death and differentiation, Vol. 14, Issue 11, pp. 1948-57, 2007

Background publications Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004

Tanida, Tanida-Miyake, Nishitani, Komatsu, Yamazaki, Ueno, Kominami: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002

Tanida, Tanida-Miyake, Komatsu, Ueno, Kominami: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002